Revisit Study of Freshwater Sponges Eunapius carteri (Bowerbank, 1863) and a New Record of Oncosclera asiatica Manconi and Ruengsawang, 2012 (Porifera: Spongillida) in Porong River, East Java, Indonesia

Distribution of freshwater sponges (Porifera: Spongillida) in Indonesia is currently insufficiently reported and underestimated compared to marine sponges. An inventory study on East Java after Indonesian independence 1945 yet be carried out. For this reason, we new records two species, Eunapius carteri (Bowerbank, 1863) Oncosclera asiatica (Manconi Ruengsawang, 2012) Porong river, Java, Indonesia. E. species was originally described Mumbai, India its presence 1927, 1928, 1930. DNA barcoding molecular phylogenetic analysis using mitochondrial COI able successfully identify our specimens as carteri, while the nuclear ITS markers placed samples with other species. Furthermore, report for first time Indonesia, O. asiatica. The from Pong River located Lower Mekong, Thailand. We concluded based morphology, skeleton, spicule composition that were similar recorded only successful obtaining sequence did not group publicly available congener, namely sp. Therefore, further taxonomy morphological needed explore diversity general conduct delimitation Asia